Last edited by Samugami
Saturday, August 15, 2020 | History

4 edition of Blood glutathione oxidation during human exercise found in the catalog.

Blood glutathione oxidation during human exercise

Blood glutathione oxidation during human exercise

  • 150 Want to read
  • 30 Currently reading

Published .
Written in English

    Subjects:
  • Glutathione,
  • Blood -- Analysis,
  • Oxygen in the body,
  • Exercise for men -- Physiological aspects

  • Edition Notes

    Statementby Christine Annette Viguie.
    The Physical Object
    FormatMicroform
    Paginationvii, 89 leaves
    Number of Pages89
    ID Numbers
    Open LibraryOL13597326M
    OCLC/WorldCa22617982

    reduced glutathione be lost by autoxidation, and (2) that the glutathione be completely recovered from the blood. In the calorimetric ferricyanide method developed by Mason (1) a tungstic acid blood filtrate is used. This method has recently been applied to human blood by two investigators, Downes.   The effects of prolonged variable-intensity and short-term high-intensity exercise on indices of oxidative stress and iron homeostasis were compared in six fit horses under cool [20°C, 40% relative humidity (RH)] or hot/humid (30°C, 80% RH) environmental conditions. The exercise protocols were designed to simulate equine competition, including racing (intense exercise Cited by:

    Effects of N-acetylcysteine on glutathione oxidation and fatigue during handgrip exercise Article in Muscle & Nerve 32(5) November with 35 Reads How we measure 'reads'. A sequential injection analysis system for determination of total and oxidized glutathione in human whole blood was reported by Araujo et al. []. .

      Rundle AG, Orjuela M, Mooney L, Tang D, Kim M, Calcagnotto A, Richie JP, Perera F. Preliminary studies on the effect of moderate physical activity on blood levels of glutathione. Biomarkers. Sep-Oct; 10(5)– Elokda AS, Nielsen DH. Effects of exercise training on the glutathione antioxidant system. Blood samples were drawn before, immediately after, and at 1, 24, and 48 h after exercise. Reduced glutathione (GSH), oxidized glutathione (GSSG), GSH/GSSG, thiobarbituric acid-reactive substances.


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Blood glutathione oxidation during human exercise Download PDF EPUB FB2

Blood samples were taken during exercise, and for up to 4 days of recovery from submaximal exercise. During exercise to VO2peak, blood reduced glutathione (GSH) and total glutathione [GSH + oxidized glutathione (GSSG)] did not change by: Blood samples were taken during exercise, and for up to 4 days of recovery from submaximal exercise.

During exercise to VO2peak, blood reduced glutathione (GSH) and total glutathione [GSH + oxidized glutathione (GSSG)] did not change significantly.

Get this from a library. Blood glutathione oxidation during human exercise. [Christine Annette Viguie]. Gohil K, Viguie C, Stanley WC, Brooks GA, Packer L: Blood glutathione oxidation during human exercise. J Appl Physiol –, PubMed Google Scholar Cited by: Blood Glutathione Oxidation during Physical Exercise We studied the effect of exhaustive physical exercise on the GSH/GSSG ratio in the blood of young male human volunteers and young male Wistar rats.4 Men performed the exercise to exhaustion on a motor-driven treadmill at increasing speed and inclination according to the Bruce protocol This protocol provides Cited by: Blood glutathione status and activities of antioxidant enzymes have been investigated during prolonged exercise with or without carbohydrate (CHO) supplementation.

Eight subjects cycled at approximately 70% of maximal oxygen uptake to fatigue [ +/- 19 (SE) min] on the first occasion (control, CON) and at the same work load and duration on Cited by: In contrast, the oxidation of red blood cell p-SHs recovered rapidly during the post-exercise period in the same manner as GSH.

The fast recovery of intra-cellular thiols during the post-exercise period might reflect the presence of reducing systems, such as glutathione by: Adipose tissue metabolically adapts to external stimuli.

We demonstrate that the induction of the thermogenic program in white adipocytes, through cold exposure in mice or in vitro adrenergic stimulation, is accompanied by a decrease in the intracellular content of glutathione (GSH).

Moreover, the treatment with a GSH depleting agent, buthionine sulfoximine (BSO), Cited by: This work reports the development of a simple, robust, automated sequential injection analysis (SIA) system for the enzymatic determination of total (tGSH) and oxidized (GSSG) glutathione in human whole blood.

The reduced (GSH) glutathione concentration is then obtained as the difference between the tGSH and GSSG by: Oxidized Forms of Glutathione in Peripheral Blood as Biomarkers of Oxidative Stress Article (PDF Available) in Clinical Chemistry 52(7).

Exhaustive physical exercise causes changes in the glutathione status of blood, liver and muscle.8, 36, 37 Moreover, glutathione status may be used as a biological marker of aging. Recently, elegant studies by Lang et al. showed considerable intra- and interindividual variability in normal GSSG levels in human blood.

Naturally, this fact could complicate interpretation of changes in blood Cited by:   Glutathione plays a central role in the maintenance of tissue antioxidant defenses and in the regulation of redox sensitive signal transduction.

In muscle cells, the level and redox status of GSH regulates activity of the redox sensitive transcription factor NF-κB.

Physical exercise may cause oxidation of GSH in tissues such as the blood, skeletal muscle and by: Blood glutathione redox ratio as a /94 $ + (94)E Original Contribution BLOOD GLUTATHIONE REDOX RATIO AS A PARAMETER OF OXIDATIVE STRESS IN PREMATURE INFANTS WITH IRDS ILONA NF-METH and DOMOKOS BODA Department of Pediatrics, Albert Szent-Gy6rgyi Medical University, Szeged, Hungary Cited by: Exercise is known to induce the oxidation of blood low-molecular-weight (LMW) thiols such as reduced glutathione (GSH).

We previously reported. Gohil et al., () showed that submaximal exercises at 65% VO 2max increased the oxidation of blood glutathione during the first 15 min of the exercise. In another study, the level of GSSG in blood increased significantly after 14 min during a maximal test in the cycle ergometer or after pedalling for 30 min in an aerobic threshold or after Cited by: 4.

What is thought to be a limiting factor of fat oxidation in muscle during higher exercise intensities decrease blood flow through adipose tissue and transport of fat acids through the mitochondria Carnitine palmitoyl transferase (CPT) is an enzyme that performs what.

So we’ve established that glutathione has huge health benefits, we can also see that glutathione is rapidly used up during intense exercise busy battling all those reactive oxygen species you are creating, and this stimulates production of more GSH.

This makes exercise a great “supplement” to boost glutathione levels. Glutathione oxidation in blood of healthy humans was first studied by Gohil et al. We tested whether oxygen therapy (F I O 2 24–26% O 2) protected against blood glutathione oxidation.

Figure 1 is a histogram showing that exercise caused a significant increase in GSSG/GSH ratio in COPD by: Limited data are available about the glutathione redox (GSSGGSH-1) status measured simultaneously in red blood cells (RBCs) and blood concerning acute high-intensity exercise.

Acute high-intensity exercise slightly increases the GSSGGSH -1 in whole blood, while GSSGGSH -1 significantly decreases in by: N-acetylcysteine pretreatment can improve performance of human limb muscle during fatiguing exercise, suggesting that oxidative stress plays a causal role in the fatigue process and identifying antioxidant therapy as a novel intervention that may be useful by:.

Thus, both in rats and humans, exhaustive physical exercise causes a change in glutathione redox status in blood. We have also found that antioxidant administration, i.e., oral vitamin C, N-acetyl-L-cysteine, or glutathione, is effective in preventing oxidation of the blood glutathione pool after physical exercise in by: Physical exercise and tissue glutathione response Human blood plasma contains low amounts of reduced glutathione (GSH).

Almost all of blood glutathione rep-resents blood cell content, mainly that of the erythrocyte. Oxidation of GSH to glutathione disulfide (GSSG) is a sensitive marker of oxidative stress.

InGohil et al.Glutathione is a substance produced naturally by the liver. It is also found in fruits, vegetables, and meats. People take glutathione by mouth for treating cataracts and glaucoma, preventing.